Knowing Tissue Culture: 

Tissue culture is a process that involves exposing plant tissue to a specific regimen of nutrients, hormones, and lights under sterile, in vitro conditions to produce many new plants, each a clone of the original mother plant, over a very short period of time. Tissue culture plants are characterised by disease free growth, a more fibrous, healthier root system, a bushier branching habit, and a higher survival rate.

There are three main steps to the tissue culture process:

1. STAGE I is the initiation phase. It concerns the establishment of plant tissue in vitro by sterilising the material and initiating it into culture. 
2. STAGE II is the multiplication phase. At this stage, the in vitro plant material is re-divided and placed in a medium with plant growth regulators that induce the proliferation of multiple shoots. This process is repeated many times until the number of plants desired is reached. 
3. STAGE III is the root formation phase. It involves the introduction of hormones to induce rooting and the formation of complete plantlets. 

Project Background: 

Kandha Project is located in Vansda Block, Navsari district of Gujarat State which has been sanctioned in the year of 2010-11 by Government of India. The project is a cluster of six micro-watersheds. The total project area of the watershed is about 6725.40 ha, of which 4000 ha has been undertaken to be treated under Integrated Watershed Management Programme (IWMP). The watershed Programme has covered seven villages namely Kandha, Bartad, Bedmal, Kamalzari, Anklachh, Khanpur and Satimal. Tribal communities are the primary inhabitants of the village.

Fig. 1 : Discussion with farmer

 Before intervention: 

Devjeebhai Jhuliabhai Deshmukh who is the beneficiary of Banana Demo Plot belongs to Bedmaal Village(Dungri Faliya). Earlier he was dependent on growing traditional crops like Paddy, Nagli, Pulses, Pigeon Pea using traditional methods. He also used to grow the traditional variety of Banana (Lokhandi variety) from which he was getting an income of Rs.30,000 to Rs.40,000 per annum. The production from one acre (o.4 ha) was nearly 4500-5000 kg. Due to non-uniform bunch formation the market price was nearly Rs 150 per mann (20kg). As a traditional banana farmer he is having a sound knowledge of banana cultivation. To make proper comparison between traditional and tissue culture farming Jhuliabhai was selected for demonstration plot.

 

IWMP intervention: 

A plot of 0.4 Ha. (one acre) was undertaken and then ploughing and levelling was done. Organic fertilizers were bought from Government Co-operative Society to increase soil fertility. Along with organic manure, chemical fertilizers were also used. The variety of Banana- Grane 9 has been planted. The cost of each plant including transportation cost is Rs.15. Thus a total of 750 Plants were bought from Gandevi Market at a total cost of Rs.11,250. These plants were planted in the month of Feb 2013. Drip Irrigation has also been installed in his field by converging with GGRC Department. The total cost of Drip was Rs.54296 out of which the contribution of Rs.19,864 was from IWMP and Rs.34,432 from GGRC respectively.

Monitoring on continuous basis was done by the project team and DWDU. MDT Agri and WDT Agri gave information on regular basis about the adoption of time to time practices. For proper cultivation of banana through tissue culture extreme care must be taken for initial three months of planting regarding timely irrigation and pesticides sprays, otherwise yield will be affected. The soils pH was also checked timely as pH more than 7.5 are not suitable for tissue culture.

Benefits of growing bananas through Tissue Culture Method to the Beneficiary:

• Plants are absolutely free from diseases and pests, so there is less field mortality. 
• Early maturity of crop- maximum land use is possible in low land holding areas. 
• Increase in yield ( nearly 2 MT per acre) 
• Produces are more uniform in shape and size, thus easily marketable with better price realisation   

 

Flow chart of processes involved:

 

After Watershed’s Intervention: 

After 9-10 months 325 mann (1 mann = 20 kg) of bananas were produced which were sold at a rate of Rs. 230 per mann. Thus after selling 325 mann he has received Rs. 74,750 as total revenue. Hence there is an increase of revenue of Rs.20000 to 25000 per acre for the farmer.

According to Devjeebhai Jhuliabhai Deshmukh, “growing bananas through tissue culture method had allowed efficient use of land and resources, realizing higher yield and net profit, easy practices, improvement in fruit quality, easy and good harvest which have ultimately lead to an increase in income”.  

Fig. 2 : Distribution of sapling	Fig. 3 : Planting of sapling
Fig. 4 : Growth stage of banana	Fig. 5 : Before harvesting

Way Forward: 

A tie-up with KVK Navsari is made to do some research on the stem part of banana for the purpose of making thread. Hence the plot is under direct supervision of KVK. With this successful intervention DWDU is now promoting tissue culture in other project areas. Various training are also being planned to generate awareness about tissue culture among the banana growers. There is a huge scope of tissue culture in near future and DWDU is acting as a dynamic partner for its promotion.

Contributors: Rashmi Sinku (Technical Expert); Rahul Vekariya (MDT Agri); Hardik Desai (MDT Agri); Rashik Ganvit (WDT Agri)